Fig. 1

Scrib KO embryos display a transient delay in epidermal permeability barrier acquisition. a Schematic representation of floxed targeting construct and mutated Scrib alleles. LoxP sites are represented by orange triangles. Cre-mediated recombination results in excision of exons 4–13, which introduces a frame-shift mutation that truncates the protein. b qRT-PCR to detect Scrib mRNA in embryonic skin at E16.5 (n = 3). Error bars: SD, *p < 0.0001 (unpaired t-test). c IF to detect Scrib (green) and DAPI (blue) in embryonic flank epidermis at E18.5 (n = 3 per genotype, scale bar = 50 μm. Inserts 1–3 scale bar = 10 μm). Scrib staining was detected along the membrane cortex of epidermal epithelial cells in Wt and Het embryonic epidermis and was absent in Scrib KO embryos. All genotypes displayed non-specific epidermal surface staining. d Representative images of toluidine blue skin permeability barrier assay (scale bar: 1 cm, n = 4–10 per genotype/time point). e H&E images of Wt, Het and KO epidermis at E16.5, E17.5 and E18.5, dashed line represents basement membrane, arrows indicate width of epidermis measured (n = 8–29 per genotype/time point, scale bar: 50 μm). f Quantitation of embryonic epidermal thickness. Error bars: SD, *p ≤ 0.0227 (2-way ANOVA with Tukey correction), n = 3 per genotype at each time point