Fig. 3

Scrib KO embryos display a transient delay in keratinocyte maturation. a Toluidine blue stained ultramicrotome sections of Scrib Wt, Het and KO embryonic flank epidermis at E16.5, E17.5 and E18.5 indicating stratum corneum (SC, orange) and stratum granulosum (SG, yellow) thickness (scale bar = 50 μm, dashed line represents basement membrane, n = 3). Quantitation of stratum corneum (b) and stratum granulosum (c) thickness in Scrib Wt, Het and KO embryonic flank epidermis at E16.5, E17.5 and E18.5 (*P ≤ 0.0066, two-way ANOVA with Tukey correction, error bars = SD, n = 3). d IF to detect K10 (green), loricrin (red) and DAPI (blue) in Scrib Wt, Het and KO embryonic flank epidermis at E16.5, E17.5 and E18.5 (scale bar = 100 μm, dashed line represents basement membrane, n = 3)