Fig. 7

Scrib depletion sensitises the DMBA/TPA-induced pre-neoplastic epidermis to papillomagenesis by inhibiting apoptosis. a Diagram illustrating short-term DMBA/TPA administration initiated at 8 weeks of age. b Representative H&E images and IHC images for BrdU, CC3, and γH2AX following short-term application of DMBA/TPA in Scrib ^+/+, Scrib ^+/fl and Scrib ^fl/fl mice (scale bar = 50 μm, n = 3). Quantitation of (c) BrdU (d) CC3 and (e) γH2AX IHC in Scrib ^+/+, Scrib ^+/fl and Scrib ^fl/fl epidermis (per 40x magnification field) that have undergone short-term treatment with either acetone, DMBA, TPA or DMBA/TPA (*p ≤ 0.0402, two-way ANOVA with Tukey correction, error bars = SD, n = 3). Acetone topical applications served as a control