Fig. 1
From: BMP4 depletion by miR-200 inhibits tumorigenesis and metastasis of lung adenocarcinoma cells
BMP4 is down-regulated in 344SQ_miR-200 cells. a The expression profile of immune-related genes in 344SQ_vector (vec) or 344SQ_miR-200 cells. Yellow: genes up-regulated by miR-200 overexpression; blue: genes down-regulated by miR-200 overexpression. b Quantitative RT-PCR (qRT-PCR) of Bmp4 and miR-200c in 13 murine lung adenocarcinoma cells. Cells were grouped into epithelial- or mesenchymal-like cells on the basis of the expression of EMT markers [13]. r and p, one-tailed Spearman’s rank correlation test. c qRT-PCR of Bmp4 in 393P, 344SQ, 344SQ_miR-200, 393P_Zeb1, and their control cells (vec). Expression levels were normalized to that of 393P (=1.0). Mean + SD, n = 3; p, two-tailed Student’s t-test. d BMP4 Western blot in 393P, 344SQ, 344SQ_vec, and 344SQ_miR-200 cells. Actin was used as a loading control. e RNA polymerase II (Pol-II) chromatin immunoprecipitation assay on the Bmp4 promoter region in 344SQ_vec and 344SQ_miR-200 cells. Bars denote DNA precipitation (% of input) from each sample. IgG was used as a negative control. Mean + SD, n = 3; p, two-tailed Student’s t-test