Fig. 6

JAG2, miR-200, and BMP4 form a regulatory loop. a qRT-PCR of Jag1, Jag2, and Bmp4 in 344SQ-NTC and Bmp4-KD cells (#2 and #3). The expression levels were normalized to those of 344SQ-NTC (=1.0). Mean + SD, n = 3; p, two-tailed Student’s t-test. b qRT-PCR of Jag1, Jag2, and Bmp4 in 344SQ-Bmp4-KD cells transfected with BMP4 expression vector and control (vec). The expression levels were normalized to those of vector-transfected groups (=1.0). Mean + SD, n = 3; p, two-tailed Student’s t-test. c qRT-PCR of Jag1, Jag2, and Bmp4 in 393P cells transfected with BMP4 expression vector and control (vec). The expression levels were normalized to those of 393P-vec (=1.0). Mean + SD, n = 3; p, two-tailed Student’s t-test. d Jag2 qRT-PCR in 393P cells treated with BMP4 protein. The expression levels were normalized to that of non-treated control (=1.0). Mean + SD, n = 3; *p < 0.01, two-tailed Student’s t-test. e, f Luciferase assay of JAG2 promoter activity in 393P cells treated with BMP4 protein (E) or co-transfected with SMAD1 and/or SMAD5 expression vectors (f). Results were normalized by using a Renilla luciferase vector (pRL-TK). Mean + SD, n = 3; p, two-tailed Student’s t-test. g qRT-PCR of Jag1, Jag2, Bmp4, and Gata3 in 344SQ cells transfected with a Jag2 shRNA (KD) or nontargeting control (NTC) vector. The expression levels were normalized to those of 344SQ-NTC (=1.0). Mean + SD, n = 3; p, two-tailed Student’s t-test. h qRT-PCR of miR-200 family members (200a, 200b, 200c, 141, and 429) in 344SQ-Jag2-KD and 344SQ-NTC. The expression levels were normalized to those of 344SQ-NTC (=1.0). Mean + SD, n = 3; p, two-tailed Student’s t-test. i qRT-PCR of miR-200 family members in 344SQ-NTC and Bmp4-KD (#2 and #3) cells. The expression levels were normalized to those of 344SQ-NTC (=1.0). Mean + SD, n = 3; p, two-tailed Student’s t-test. j Diagram of a regulatory loop involving BMP4, JAG2, and miR-200