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Fig. 1 | Molecular Cancer

Fig. 1

From: Aryl hydrocarbon receptor-microRNA-212/132 axis in human breast cancer suppresses metastasis by targeting SOX4

Fig. 1

TCDD and DIM inhibit motility of breast cancer cells in vitro in an Ahr-dependent fashion. a The inhibitory effects of 10 nmol/L TCDD or 25 μmol/L DIM on migration of MDA-MB-231 and expansion of T47D cells were examined by wound healing assay. b Cell invasion of MDA-MB-231 was suppressed by TCDD (10–25 nmol/L) and DIM (10–50 μmol/L) in the Boyden chamber assay. c DIM (10–25 μmol/L), but not TCDD, inhibited the invasion of T47D cells. d Efficiency of Ahr knockdown by siAhr compared with siNS was confirmed by real-time PCR and western blot. e siAhr abrogated the inhibitory effects of TCDD and DIM on the invasion of MDA-MB-231 and T47D cells in the Boyden chamber. f Activation of Ahr by TCDD (10 nmol/L) and DIM (25 μmol/L) was confirmed by quantification of CYP1A1 gene expression by real-time PCR and western blot. Data are shown as mean ± SD of three independent experiments performed in triplicates. b, c and f *; P < 0.05, significantly different from DMSO-treated control. d *; P < 0.05, significantly different from siNS-transfected control. e significantly different from DMSO- and siNS-treated control

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