Fig. 2

TCDD and DIM induce miR-212/132 cluster in breast cancer cells in an Ahr-dependent fashion. a TCDD (1–25 nmol/L) and DIM (10–50 μmol/L) induced miR-212/132 cluster in MDA-MB-231 and T47D cells, miRNA expression was quantified by real-time PCR. b siAhr blocked the 10 nmol/L TCDD- and 25 μmol/L DIM-induced miR-212/132 expression in MDA-MB-231 and T47D cells compared with the siNS-transfected control. c ChIP assay analysis of Ahr binding activity on XRE box on upstream sequence of miR-212/132 gene. The breast cancer cells were treated with 10 nmol/L TCDD or 25 μmol/L DIM for 24 h. Presented is XRE-2 that showed interaction with Ahr. d siAhr suppressed the luciferase activity of miR-212/132 promoter reporter. Data are shown as mean ± SD of three independent experiments performed in triplicates. a *P < 0.05, significantly different from DMSO-treated control. b and d *P < 0.05, significantly different from DMSO-treated control or Ahr agonist- and siAhr-treatments