Fig. 3

MiR-212/132 cluster has a direct inhibitory role on motility of breast cancer cells in vitro. The role of miR-212/132 cluster on migration of MDA-MB-231, expansion of T47D and invasion of breast cancer cells were examined by the transfection of miRNA mimics, miR-132 and miR-212, or antisense, as-miR-132, as-miR-212. a Over-expression of the miR-212/132 cluster by mimics suppressed the migration of MDA-MB-231 and expansion of T47D cells in wound healing assay compared with siNS-transfected control. b Mimics of the miR-212/132 cluster suppressed the invasion of MDA-MB-231 and T47D cells in the Boyden chamber assay. c Inhibition of miR-212/132 cluster by antisense partially blocked the inhibitory effects of TCDD and DIM on invasion of MDA-MB-231 and T47D cells. d Efficiency of miR-212/132 knockdown by antisense compared with siNS was confirmed by real-time PCR. Data are shown as mean ± SD of three independent experiments performed in triplicates. b and d *P < 0.05, significantly different from siNS-transfected control. c *P < 0.05, significantly different as shown by lines