Fig. 5

TCDD and DIM suppress metastasis of breast cancer cells to the lungs and induce miR-212/132 in vivo. MDA-MB-231 or T47D were injected in the fat pad of the second nipple of athymic nude mice. TCDD (25 μg/kg/day), DIM (50 mg/kg/day), or corn oil (Veh) were given orally for 10 days. Non-transplanted (Neg) mice were used as negative controls. a DIM treatment decreased the weight of T47D primary tumor that formed at the breast cancer cell injection site. b TCDD and DIM treatments decreased the number of pulmonary nodules in MDA-MB-231-injected mice, while the number of the nodules in T47D-injected mice was decreased with DIM treatment. c TCDD and DIM induced the miR-212/132 expression in pulmonary nodules of MDA-MB-231- or T47D-injected mice. d TCDD treatment suppressed SOX4 mRNA in the pulmonary nodules of MDA-MB-231-injected mice. Data are shown as mean ± SD of three independent experiments. n = 8 in MDA-MB-231-injected mice. a–d *P < 0.05, significantly different from Veh-treated control