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Table 1 Primer sequences and PCR reaction conditions

From: Epigenetic silencing of a long non-coding RNA KIAA0495 in multiple myeloma

Primer set Forward primer (5’ – 3’) Reverse primer (5’ – 3’) Product size (bp) MgCl2/Tm/cycles Reference
(I) Methylation-specific polymerase chain reaction (MSP)    
KIAA0495      
M-MSP TGG AGA TAA CGG GTT TAG AGA AAT C AAC GAA AAC AAA AAT AAA ACC TTC G 191 1.5 mM/58 °C/40X --
U-MSP TGG AGA TAA TGG GTT TAG AGA AAT T AAA CAA AAA CAA AAA TAA AAC CTT CA 192 2.0 mM/58 °C/40X --
(II) Reverse transcription-polymerase chain reaction (RT-PCR)    
KIAA0495 GCT GCT TGC TGT ACG TGG TG CGT GGC TGA CAC AAA CTT GC 178 1.5 mM/60 °C/35X [16]
GAPDH ACC ACA GTC CAT GCC ATC ACT TCC ACC ACC CTG TTG CTG TA 452 1.5 mM/60 °C/24X  
  1. Keys: Tm, annealing temperature; M-MSP, MSP for the methylated allele; U-MSP, MSP for the unmethylated allele