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Fig. 4 | Molecular Cancer

Fig. 4

From: Downregulation of ASPP2 in pancreatic cancer cells contributes to increased resistance to gemcitabine through autophagy activation

Fig. 4

ASPP2 regulates autophagy through AMPK-mTOR pathways. a Indicated proteins were detected with immunoblots in BxPC-3 cells with stable ASPP2 and SW1990 cells with ASPP2 stably knockdown. b Identification of shRNA activity of AMPK with RT-PCR and immunoblots. c sh-con/sh-ASPP2 SW1990 cells with GFP-LC3 were transfected with sh-Con/sh-AMPK, autophagosomes formation was observed under microscopy. The percentage of autophagic cells was calculated in 5 random fields. d Indicated molecules were detected with immunoblots in sh-con/sh-ASPP2 SW1990 cells transfected with sh-Con/sh-AMPK. e sh-con/sh-ASPP2 SW1990 cells were transfected with sh-Con/sh-AMPK, then infected with adenovirus-delivering mRFP-GFP-LC3. Quantification of autophagosome and autolysosome formation represents punctures staining sites per cell of 5 independent images. Data represent the mean ± SD of three independent experiments (*P < 0.05, **P < 0.01)

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Molecular Cancer

ISSN: 1476-4598