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Fig. 5 | Molecular Cancer

Fig. 5

From: Downregulation of ASPP2 in pancreatic cancer cells contributes to increased resistance to gemcitabine through autophagy activation

Fig. 5

ASPP2 can enhance gemcitabine induced cell apoptosis. a The surviving rate of cancer cells were detected with CCK-8 assays in BxPC-3 cells with stable ASPP2 and SW1990 cells with ASPP2 stably knockdown treated with gemcitabine (1 μM) for indicated times.b the rate of apoptosis cells were determine by Annexin V-FITC Apoptosis Detection Kit in cells of (A). c The indicated proteins were detected with immunoblots in BxPC-3 cells with stable ASPP2 and SW1990 cells with ASPP2 stably knockdown treated with gemcitabine (1 μM) for 48 h. d,e the indicated molecules, The surviving rate of cancer cells and the rate of apoptosis cells were detected in BxPC-3 cells with stable ASPP2 treated with gemcitabine (1 μM) or gemcitabine (1 μM) plus pifithrin-α (10 μM). Data represent the mean ± SD of three independent experiments (*P < 0.05, **P < 0.01)

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Molecular Cancer

ISSN: 1476-4598