Fig. 5

c-Myc-induced over-expression of pri-miR-17-92a was attenuated by butyrate treatment. HCT116 human colon cancer cells were infected with adenovirus carrying the c-Myc coding sequence driven by a CMV promoter to overexpress c-Myc (cMyc) or a null virus vehicle (Veh) for 48 h before analysis. Cells without virus infection were analyzed as control (Cont). The effects of treatment with 2 mM butyrate was assessed using three experimental designs: a no butyrate treatment, b 24-h treatment prior to cell harvest, and c) 56-h treatment starting 8 h prior to virus infection. Untreated cells (UNTD) were assessed as control. Protein levels of c-Myc and β-actin were analyzed by immunoblotting. Images shown are representative of three individual experiments. d The abundance of pri-miR-17-92a was measured using qPCR. Bars represent means ± SEM. *P < 0.05, n = 3