Fig. 2
From: Alcohol-dysregulated miR-30a and miR-934 in head and neck squamous cell carcinoma
Ethanol and acetaldehyde treatment in normal culture cells verifies clinical data analysis of alcohol-dysregulated microRNAs. (a) qRT-PCR demonstrates that long-term ethanol treatment of normal oral epithelial cells upregulates miR-30a and miR-934 in OKF4 and OKF6. (b) qRT-PCR demonstrates that 48-hour acetaldehyde treatment of normal oral epithelial cells also upregulates miR-30a and miR-934 in OKF4 and OKF6. (c) Long-term ethanol treatment also upregulates 4 other alcohol-associated miRNAs in OKF4 and OKF6. (d) Acetaldehyde treatment also upregulates 4 other alcohol-associated miRNAs in OKF4 and OKF6. (e) 1- and 2-week exposure of UMSCC-10B and UMSCC-22B results in upregulation of the candidate miRNAs, as does (f) 48-hour acetaldehyde treatment of the two HNSCC cell lines. All data is presented as the mean and error bars represent standard deviation. *p< 0.05, **p<0.01, ***p<0.001, Student’s t-test