Fig. 4

Inhibition of miR-30a and miR-934 decreases cancer stem cell genes, enhances cisplatin-induced cell death, and decreases invasive abilities in HNSCC cell lines. (a) qRT-PCR demonstrates that miR-30a and miR-934 are significantly upregulated in HNSCC cell lines, UMSCC-10B and UMSCC-22B, compared to the normal cell line, OKF4. (b) Knockdown of baseline expression in HNSCC cell lines was measured by qRT-PCR, resulting in75% knockdown or greater in the cell lines for both miRNAs. (c) MTS assay depicts that the knockdown of miR-30a and miR-934 sensitizes the HNSCC cell lines to cisplatin as shown by the reduction in cisplatin IC-50. IC-50 values were obtained by fitting a dose–response curve to the averaged values for relative proliferation under each condition and cisplatin dose. (d) Matrigel invasion assay shows a decrease in the number of invading cells after knockdown of miR-30a and miR-934 relative to control. All data is presented as the mean and error bars represent standard deviation. *p<0.05, **p<0.01, ***p<0.001, Student’s t-test