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Fig. 3 | Molecular Cancer

Fig. 3

From: A20 suppresses hepatocellular carcinoma proliferation and metastasis through inhibition of Twist1 expression

Fig. 3

A20 suppresses proliferation and tumor growth of HCC cells in vitro and in vivo. a Stable knockdown or overexpression HCCLM3 cells (LM3-shA20 and LM3-A20, respectively) and their empty vector counterparts (LM3-shcon and LM3-con, respectively) were subjected to Western blot assay and probed with specific antibodies to A20 and β-actin. b Cell viability was determined by the Brdu cell proliferation assay at indicated times. Plots are mean ± SEM of data from three independent experiments. *, p < 0.05. c Xenograft tumor model assays. 2x105 of LM3-shcon or LM3-shA20 cells were subcutaneously injected into the bilateral flanks of nude mice. Representative images of the subcutaneous xenografts are shown (left upper panel). Subcutaneous xenografts from the LM3-shcon and LM3-shA20 groups were excised from nude mice (n = 4) (left down panel). The growth curves of xenografts tumor volumes (right panel). *, p < 0.05. d 2 × 105 of LM3-con or LM3-A20 cells were subcutaneously injected into the bilateral flanks of nude mice. Representative images of the subcutaneous xenografts are shown (left upper panel). Subcutaneous xenografts from the LM3-con and LM3-A20 groups were excised from nude mice (n = 4) (left down panel). The growth curves of xenografts tumor volumes (right panel). *, p < 0.05. e-f Indicated xenograft tumors were subjected to H&E and immunohistochemical staining of A20 and PCNA. Representative images are shown. The scale bars represent 50 μm

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