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Fig. 6 | Molecular Cancer

Fig. 6

From: A20 suppresses hepatocellular carcinoma proliferation and metastasis through inhibition of Twist1 expression

Fig. 6

Decreased NF-κB activity is involved in A20-induced downregulation of Twist1. a-b Indicated cells were cotransfected with NF-κB promoter-luc (0.5ug/well) and pRL-TK plasmids (20 ng/well). After 24 h of transfection, cells were serum starved overnight followed by treated with or without TNFα (10 ng/mL) for additional 12 h, and cell lysates were prepared to perform a reporter gene assay. Plots are mean ± SEM of data from three independent experiments. *, p < 0.05. c Cells were serum starved overnight followed by treated with TNFα (10 ng/mL) for the indicated times and then cell lysates were harvested and subjected to Western blot assay and probed with the indicated antibodies. d Cells were transfected with IκBα or empty vector plasmid for 24 h and then cell lysates were harvested and subjected to Western blot assay and probed with the indicated antibodies

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