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Fig. 4 | Molecular Cancer

Fig. 4

From: Poly(A)-specific ribonuclease and Nocturnin in squamous cell lung cancer: prognostic value and impact on gene expression

Fig. 4

Alterations of gene expression in NCI-H520 and Hep2 cells upon PARN and NOC silencing. a, b Comparison of gene expression alterations between NCI-H520 and Hep2 cells upon PARN (a) and NOC (b) silencing. Numbers indicate up- and downregulated transcripts. c-e Expression of PTGIR, AATK and DPPA3 mRNAs in NCI-H520 cells upon PARN or NOC silencing. The abundance of PTGIR mRNA was determined in wild type (WT) and PARN knock down (T-P) cells by RT-qPCR followed by analysis of the reaction products on 2 % agarose gel and normalized to b-actin mRNA (c). The expression levels of AATK (d) and DPPA3 (e) mRNAs were determined in T-P and NOC knock down (T-N) cells by RT-qPCR and compared to those of WT cells. L, DNA molecular mass marker; numbers on the right indicate DNA base pairs (bp). Error bars represent the standard deviation

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