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Table 2 Transcriptional modulation in GSC by BMP7 treatmenta

From: Bone morphogenetic protein 7 sensitizes O6-methylguanine methyltransferase expressing-glioblastoma stem cells to clinically relevant dose of temozolomide

Gene

Fold change

P value

Functional involvement

I. Upregulated genes

   

RASAL2: RAS protein activator like 2

3.37

0.019083

tumor and metastasis suppressor

ATXN1: ataxin 1

2.59

0.013118

chromatin-binding factor that repress Notch signaling, cell differentiation

CD44: CD44 molecule (Indian blood group)

2.59

0.023871

a receptor for hyaluronic acid; modulates Smad1 activation in the BMP7 signaling

ZNF638: protein 638

2.33

0.030429

adipocyte differentiation

CDC14B: CDC14 cell division cycle 14 homolog B (S. cerevisiae)

2.09

0.027588

cell cycle control

TGFBR1: Transforming growth factor, beta receptor I

1.99

0.015786

cell cycle arrest in epithelial cells; growth inhibition, cell apoptosis

PIK3C2A: Phosphoinositide-3-kinase, class 2, alpha polypeptide

1.63

0.037372

functions in insulin signaling, premature senescence and oxidative apoptosis

AKAP10: A kinase (PRKA) anchor protein 10

1.59

0.024294

control of cell proliferation

DLC1: deleted in liver cancer 1

1.58

0.041191

metastasis suppressor gene, EMT arrest

BRPF3: bromodomain and PHD finger containing, 3

1.44

0.035113

promotes formation of acetyltransferase complexes, stimulates transcription

SLC20A1: Solute carrier family 20 (phosphate transporter), member 1

1.40

0.042665

cellular metabolism, signal transduction, and nucleic acid and lipid synthesis

RASEF: RAS and EF-hand domain containing

1.39

0.045602

tumor suppressor, induction of apoptosis, inhibition of proliferation

MLL: myeloid/lymphoid or mixed-lineage leukemia

1.38

0.024547

regulates neural progenitor proliferation and neuronal and glial differentiation

CDK6: cyclin-dependent kinase 6

1.37

0.047985

augments accumulation of p53, decreases tumor growth when overexpression

IGF1R: insulin-like growth factor 1 receptor

1.34

0.045232

stimulates stem cell differentiation via AKT activation; premature senescence

POU2F1: POU class 2 homeobox 1

1.34

0.032549

anti-inflammation/immune response, cell apoptosis, regulation of NF-kappaB

IL6ST: interleukin 6 signal transducer (gp130, oncostatin M receptor)

1.29

0.022673

stimulates osteoblast differentiation, maintains bone formation

ZNF395: zinc finger protein 395

1.29

0.030385

suppresses cell migration and invasion

ERN2: endoplasmic reticulum to nucleus signaling 2

1.28

0.037335

activation of the unfolded protein response

LGR5: leucine-rich repeat-containing G protein-coupled receptor 5

1.27

0.030355

universal epithelial stem cell marker

IRAK1BP1: interleukin-1 receptor-associated kinase 1 binding protein 1

1.27

0.024291

downregulation of inflammatory cytokines, anti-inflammation, growth inhibition

ARIH2: Ariadne homolog 2 (Drosophila)

1.26

0.032696

inhibits cell proliferation

II. Downregulated genes

   

NOTCH3: Notch homolog 3 (Drosophila)

−1.25

0.037102

EMT, maintenance of NSC in an undifferentiated quiescent state, angiogenesis

ASXL1: additional sex combs like 1 (Drosophila)

−1.25

0.016868

suppresses adipogenesis, transcriptional repression

SEMA6A: sema, transmembrane, and cytoplasmic domains, 6A

−1.25

0.041608

vascular development; tumor angiogenesis; neuronal development

QPRT: quinolinate phosphoribosyltransferase

−1.25

0.027582

resistance to oxidative stress; suppression of spontaneous cell death

GPRC5B: G protein-coupled receptor, family C, group 5, member B

−1.26

0.029052

insulin resistance; inflammatory signaling; promotes ERK1/2 activation

TRO: trophinin

−1.26

0.033855

promotes EMT, invasive and metastatic phenotype; tumor formation

MFI2: antigen p97 identified by mAb 133.2 and 96.5

−1.26

0.034824

cell proliferation and tumorigenesis

METTL3: methyltransferase like 3

−1.28

0.046969

plays a role in the efficiency of mRNA splicing and processing

MTMR11: myotubularin related protein 11

−1.35

0.017165

unknown; cisplatin resistance-associated protein

COL16A1: collagen, type XVI, alpha 1

−1.37

0.020495

stimulates tumor proliferation and invasion

SCARA5: Scavenger receptor class A, member 5 (putative)

−1.40

0.024406

innate immune response; inflammatory response

CLEC7A: C-type lectin domain family 7, member A

−1.40

0.009915

innate immune response; inflammatory response; migration and proliferation

  1. NSC neural stem cells; EMT epithelial-to-mesenchymal transition
  2. aProbe set signals on the expression array that were ≥ 1.25-fold different in BMP7 treated GSC cultures (n = 2, GSC-parental and GSC-500 μM TMZ) when compared to untreated GSC cultures (n = 2, GSC-parental and GSC-500 μM TMZ) were selected