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Table 2 Transcriptional modulation in GSC by BMP7 treatmenta

From: Bone morphogenetic protein 7 sensitizes O6-methylguanine methyltransferase expressing-glioblastoma stem cells to clinically relevant dose of temozolomide

Gene Fold change P value Functional involvement
I. Upregulated genes    
RASAL2: RAS protein activator like 2 3.37 0.019083 tumor and metastasis suppressor
ATXN1: ataxin 1 2.59 0.013118 chromatin-binding factor that repress Notch signaling, cell differentiation
CD44: CD44 molecule (Indian blood group) 2.59 0.023871 a receptor for hyaluronic acid; modulates Smad1 activation in the BMP7 signaling
ZNF638: protein 638 2.33 0.030429 adipocyte differentiation
CDC14B: CDC14 cell division cycle 14 homolog B (S. cerevisiae) 2.09 0.027588 cell cycle control
TGFBR1: Transforming growth factor, beta receptor I 1.99 0.015786 cell cycle arrest in epithelial cells; growth inhibition, cell apoptosis
PIK3C2A: Phosphoinositide-3-kinase, class 2, alpha polypeptide 1.63 0.037372 functions in insulin signaling, premature senescence and oxidative apoptosis
AKAP10: A kinase (PRKA) anchor protein 10 1.59 0.024294 control of cell proliferation
DLC1: deleted in liver cancer 1 1.58 0.041191 metastasis suppressor gene, EMT arrest
BRPF3: bromodomain and PHD finger containing, 3 1.44 0.035113 promotes formation of acetyltransferase complexes, stimulates transcription
SLC20A1: Solute carrier family 20 (phosphate transporter), member 1 1.40 0.042665 cellular metabolism, signal transduction, and nucleic acid and lipid synthesis
RASEF: RAS and EF-hand domain containing 1.39 0.045602 tumor suppressor, induction of apoptosis, inhibition of proliferation
MLL: myeloid/lymphoid or mixed-lineage leukemia 1.38 0.024547 regulates neural progenitor proliferation and neuronal and glial differentiation
CDK6: cyclin-dependent kinase 6 1.37 0.047985 augments accumulation of p53, decreases tumor growth when overexpression
IGF1R: insulin-like growth factor 1 receptor 1.34 0.045232 stimulates stem cell differentiation via AKT activation; premature senescence
POU2F1: POU class 2 homeobox 1 1.34 0.032549 anti-inflammation/immune response, cell apoptosis, regulation of NF-kappaB
IL6ST: interleukin 6 signal transducer (gp130, oncostatin M receptor) 1.29 0.022673 stimulates osteoblast differentiation, maintains bone formation
ZNF395: zinc finger protein 395 1.29 0.030385 suppresses cell migration and invasion
ERN2: endoplasmic reticulum to nucleus signaling 2 1.28 0.037335 activation of the unfolded protein response
LGR5: leucine-rich repeat-containing G protein-coupled receptor 5 1.27 0.030355 universal epithelial stem cell marker
IRAK1BP1: interleukin-1 receptor-associated kinase 1 binding protein 1 1.27 0.024291 downregulation of inflammatory cytokines, anti-inflammation, growth inhibition
ARIH2: Ariadne homolog 2 (Drosophila) 1.26 0.032696 inhibits cell proliferation
II. Downregulated genes    
NOTCH3: Notch homolog 3 (Drosophila) −1.25 0.037102 EMT, maintenance of NSC in an undifferentiated quiescent state, angiogenesis
ASXL1: additional sex combs like 1 (Drosophila) −1.25 0.016868 suppresses adipogenesis, transcriptional repression
SEMA6A: sema, transmembrane, and cytoplasmic domains, 6A −1.25 0.041608 vascular development; tumor angiogenesis; neuronal development
QPRT: quinolinate phosphoribosyltransferase −1.25 0.027582 resistance to oxidative stress; suppression of spontaneous cell death
GPRC5B: G protein-coupled receptor, family C, group 5, member B −1.26 0.029052 insulin resistance; inflammatory signaling; promotes ERK1/2 activation
TRO: trophinin −1.26 0.033855 promotes EMT, invasive and metastatic phenotype; tumor formation
MFI2: antigen p97 identified by mAb 133.2 and 96.5 −1.26 0.034824 cell proliferation and tumorigenesis
METTL3: methyltransferase like 3 −1.28 0.046969 plays a role in the efficiency of mRNA splicing and processing
MTMR11: myotubularin related protein 11 −1.35 0.017165 unknown; cisplatin resistance-associated protein
COL16A1: collagen, type XVI, alpha 1 −1.37 0.020495 stimulates tumor proliferation and invasion
SCARA5: Scavenger receptor class A, member 5 (putative) −1.40 0.024406 innate immune response; inflammatory response
CLEC7A: C-type lectin domain family 7, member A −1.40 0.009915 innate immune response; inflammatory response; migration and proliferation
  1. NSC neural stem cells; EMT epithelial-to-mesenchymal transition
  2. aProbe set signals on the expression array that were ≥ 1.25-fold different in BMP7 treated GSC cultures (n = 2, GSC-parental and GSC-500 μM TMZ) when compared to untreated GSC cultures (n = 2, GSC-parental and GSC-500 μM TMZ) were selected