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Fig. 6 | Molecular Cancer

Fig. 6

From: PKM2 regulates neural invasion of and predicts poor prognosis for human hilar cholangiocarcinoma

Fig. 6

Treatment with metformin inhibited HC cell proliferation and tumor growth via suppression of PKM2 and SDC2 expression. a, QBC939 cells was treated with metformin at different concentrations (0, 10, 20, and 50 mM), and their viability was measured at 24 and 72 h after treatment using an MTT assay. b, Western blotting was used to determine the level of PKM2 and SDC2 expression after treatment with metformin. PBS, phosphate-buffered saline. c and d, in situ tumor xenograft models were used to assess the inhibitory effect on tumor growth of treatment with metformin (250 mg/kg) compared with that in a negative control. Tumor weights (c) and representative tumors from each group (d) were measured at the end of the experiment. e, PKM2, SDC2, and PCNA were detected in the xenograft tumors using immunohistochemistry

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