Fig. 3

Effects of long-term TQ treatment on NF-κB activity in tumors. a Western blot analysis of p65 in nuclear extracts from ID8-NGL tumors harvested from mice treated with thrice weekly 20 mg/kg TQ or vehicle (Veh) for 30 days. Equal loading was shown by probing for the nuclear-specific protein, histone H3. b Nuclear p65 expression relative to corresponding histone H3 levels was measured by densitometry. c Immunofluorescent detection of the proliferation marker Ki67/mib-1 and the phosphorylated p65 (p-p65). DAPI-stained nuclei are in blue. Co-expression of phospho-p65 (p-p65; red) and Ki67/mib-1 (green) is observed in the nuclei of a subset of cells. d The percentage of tumor cells positive for Ki67 staining or nuclear p-p65, and the percentage of Ki67-positive cells also expressing nuclear p-p65. At least 200 cells were counted in 5 independent fields (×40 objective) for each drug treatment per mouse. Values are mean + SD for 3 mice per group *p < 0.01 relative to vehicle-treated mice, Mann–Whitney test