Fig. 6

Macrophage infiltration in tumors in TQ-treated mice. BL/6 mice were injected with ID8-NGL cells and treated with vehicle, TQ (20 mg/kg), cisplatin (2 mg/kg) or the combination. a IF analysis of expression of the macrophage markers F4/80 (green) and arginase-1 (red) in paraffin-embedded tumor sections. DAPI-stained nuclei are in blue. b Quantification of the percentage of total cells positive for F4/80 and cells co-staining for F4/80 and arginase-1. Values were determined from 5 representative fields at high power (×40). Values are mean + SE for 3–5 mice per group. *p < 0.01 relative to vehicle; all p values determined by Mann–Whitney test