Fig. 9

Increased pro-tumorigenic inflammatory mediators in long-term TQ-treated mice. a QPCR measurement of mRNA levels of the established NF-κB target genes, IL-1β and TNF-α, in harvested peritoneal macrophages from mice treated with thrice weekly 20 mg/kg TQ or vehicle (Veh) for 10 or 30 days. Values were expressed relative to corresponding GAPDH levels. b VEGF levels in ascites fluid was measured by ELISA and expressed relative to corresponding protein levels. Values are mean + SE for 3–5 mice per group. *p < 0.01 relative to vehicle; all p values determined by Mann–Whitney test. c Cytokine/growth factor profiles in ascites from vehicle or TQ treated mice (30 days)