Fig. 2

Inhibition of miR-362-5p suppresseed cell proliferation, induced cell apoptosis and cell cycle arrests during G1 phase, in both BV173 and K562 cells. BV173 and K562 cells were either left untransfected or were transfected with either a miR-362-5p inhibitor (inhibitor) or a miR-362-5p inhibitor control (inhibitor-ctrl). a Relative expression of miR-362-5p was analysed by qRT-PCR. U6snRNA was used as an internal control. b Proliferation of BV173 and K562 cells was using the CCK-8 assay. c CML cell lines were stained with PI. The cell cycle distribution was analysed by FACS. d CML cell lines (in the absence or presence of 5 μg/ml Ara-c) were stained with PI and Annexin V. Early and late apoptotic cells are shown in the right quadrant. *P < 0.05,**P < 0.01