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Fig. 1 | Molecular Cancer

Fig. 1

From: Dasatinib blocks transcriptional and promigratory responses to transforming growth factor-beta in pancreatic adenocarcinoma cells through inhibition of Smad signalling: implications for in vivo mode of action

Fig. 1

Immunoblot analysis of TGF-β1-induced R-Smad activation in Panc-1 and Colo-357 cells treated with dasatinib. Panc-1 and Colo-357 cells were stimulated with 5 ng/ml TGF-β1 for 1 h in the presence of vehicle (DMSO, 0.1 % v/v) or the indicated concentrations of dasatinib, and subsequently evaluated by immunoblotting for C-terminal phosphorylation of Smad2 (p-Smad2C, upper two panels) and Smad3 (p-Smad3C, lower two panels). Equal loading of total protein was verified with antibodies to Smad2 and Smad3, respectively. Below the immunoblots is a graphic representation of the results from densitometric analysis of p-Smad band intensities after normalization with those for the respective total Smad proteins

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