Fig. 5
Effect of dasatinib on TGF-β1-induced cell migration in PDAC cell lines. Overnight starved Panc-1 or Colo-357 cells were resuspended in growth medium with 1 % FCS and seeded into the wells of a CIM-Plate 16 (60,000/well) of the RTCA DP instrument. Cells were allowed to migrate in the presence of TGF-β1 (5 ng/ml, added to both the lower and upper compartment of each well) and either vehicle or dasatinib at the indicated concentrations (a & b) or SB431542 (c) as indicated by the colour code. Changes in impedance resulting from cells that have migrated to the bottom side of the membranes were recorded every 15 min and monitored for a total of 12 h (Panc-1) and 24 h (Colo-357). Data are from one representative experiment out of three experiments performed in total and are presented as means ± SD of quadruplicate wells. In each graph, significance was calculated for vehicle + TGF-β1 vs. dasatinib + TGF-β1 (a & b) or vs. SB431542 + TGF-β1 (c). The first time point at which differences become significant are for Panc-1 cells: 10 μM: 0:30 (a, left-hand graph); 1 μM: 0:15; 0.1 μM: 0:15; 0.01 μM: 0:30 (b, left-hand graph); 5 μM: 0:30 (c, left-hand graph); and for Colo-357 cells: 10 μM: 0:47 (a, right-hand graph); 1 μM: 1:04; 0.1 μM: 3:50; 0.01 μM: 4:35 (b, right-hand graph); 5 μM: 0:30 (c, right-hand graph)