Skip to main content
Fig. 6 | Molecular Cancer

Fig. 6

From: Dasatinib blocks transcriptional and promigratory responses to transforming growth factor-beta in pancreatic adenocarcinoma cells through inhibition of Smad signalling: implications for in vivo mode of action

Fig. 6

Dasatinib inhibits cell migration induced by kinase-active ALK5. Cells (60,000/well) from a previously characterised clone of Panc-1-ALK5T204D cells were seeded into a CIM-Plate 16 for measurement of cell migration. Cells were allowed to migrate in the absence or presence of exogenous TGF-β1 (5 ng/ml) and either DMSO (0.1 %) or dasatinib (10 μM) (a) or SB431542 (5 μM) (b). Data shown are given as mean ± SD of quadruplicate wells and were derived from one representative experiment out of three experiments performed in total. Significant differences in the cell index values between vehicle treated (red curve) and dasatinib-treated (green curve) Panc-1-ALK5TD cells, and between vehicle + TGF-β1 treated (blue curve) dasatinib + TGF-β1-treated (ochre curve) were first observed at the 1:00 h and all later time points

Back to article page