Fig. 1

The level of EXNs increases after irradiation or chemotherapy, and EXNs induce migration and adhesion of lung cancer cell lines. Panels a and b. The level of ATP, UTP and adenosine in BM lysate (Panel a) and CM from BM cells (Panel b) prepared from animals 24 h after irradiation (0–1500 cGy) or vincristine administration (0.5–2 mg/kg). Measurements were performed for samples from three (ATP) or two (UTP and adenosine) independent isolations. Each analysis was performed in duplicates. Panel c. Dose-dependent chemotactic response of the HTB177 cell line to ATP (left) and hepatocyte growth factor (HGF) at physiological (L; 0.3 ng/ml) or supraphysiological (H; 10 ng/ml) concentrations (right). Panel d. Optimal doses of the tested nucleotides based on chemotactic dose-dependent responses of lung cancer cell lines tested with ATP, ADP, AMP, and adenosine. Panel e. Chemotaxis of NSCLC and SCLC cells in response to ATP, ADP, AMP, and adenosine and a supraphysiological dose of HGF (10 ng/ml). Experiment was performed at least three times. Panel f. Adhesion of NSCLC and SCLC cells to fibronectin after stimulation with ATP, ADP, AMP, and adenosine. Experiment was performed three times. *p < 0.05