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Fig. 3 | Molecular Cancer

Fig. 3

From: Extracellular nucleotides as novel, underappreciated pro-metastatic factors that stimulate purinergic signaling in human lung cancer cells

Fig. 3

P1 and P2 receptors are functional in lung cancer cell lines. Panels a-b. Phosphorylation of p42/44 MAPK and AKT in the human NSCLC cell line HTB177 (Panel a) and the SCLC cell line CRL5853 (Panel b) after being stimulated for 5 min with the indicated nucleotides. The experiment was repeated twice, with similar results, and representative western blots are shown. Right panels show quantitative analysis of MAPKp42/44 and AKT phosphorylation. Panel c. Intracellular calcium measurments in lung cancer cell lines in response to 100 μM ATP (left top) or 1 mM ATP (right top), 100 μM Bz-ATP (left bottom), or 100 μM adenosine (right bottom). Analysis was performed at least 3 times in triplicates. Values are mean ± SEM

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