Fig. 1

Expression of P2X7R in PDACs and HPDE cells. a. Real time PCR and RT-PCR analysis of P2X7R expression in HPDE and PDAC cells. Insert shows a representative gel of P2X7R mRNA (284 bp) in Panc-1. The data were normalized with respect to the three housekeeping genes: β-actin, β glucuronidase (GUSB) and glutaminyl-tRNA synthetase (QARS) and expression in HPDE was set to 1. The bargraph shows data for four experiments (mean ± SEM). The relative amount of mRNA was calculated from a standard curve run on each plate. b. Western blot band at around 70 kDa corresponding to the isoform A [PR: Q99572-1], as shown on the insert. Loading control was β-actin [PR: P60709] detected at 42 kDa. All lanes were loaded with 50 μg of proteins. c. Western blot band at around 42 kDa most likely corresponds to the isoform B [PR: Q99572-2]. The results were normalized with β-actin that was used as a loading control. The graphs for the two isoforms show data of three experiments (mean ± SEM). Significant differences of expression in PDAC in comparison to HPDE cells P < 0.05 (*) and P < 0.001 (**) are indicated