Fig. 5

Effect of ATP and A438079, KN-62 on BrdU incorporation in Panc-1 and BxPC-3. Data show the BrdU incorporation in both cell lines after 60 h. The colored columns show the effect of different concentrations of added (exogenous) ATP (100 μM, 1 mM and 5 mM). The white columns show the effect of ATP and control (no exogenous ATP added) in combination with the two inhibitors A438079 (10 μM) or KN-62 (100 nM), which were added 1 h before ATP. The data were normalized to 1 % serum control (100). The graphs show data from three to six experiments (mean ± SEM); where each run was carried out in triplicates. Significant differences P < 0.05 (*, #) and P < 0.001 (**) from the respective 1 % serum control (*, **) and with/without inhibitor (#) are indicated