Fig. 6

Cell death in Panc-1 cells in the presence of 1 mM ATP. a. Caspase 3/7 activation was measured in control cells and after 20 h incubation with 1 mM ATP, AZ10606120 (10 μM), combination of both, and AT101 (apoptosis inducer). The results were normalized to 1 % serum control (1.0). The graph shows data from four experiments (mean ± SEM); each run was carried out in duplicates. Significant difference P < 0.001 (**) from the respective 1 % serum control is indicated. b. Annexin V-488 (green) and propidium iodide (red) were added after 20 h incubation with 1 mM ATP in 1 % of serum. Fluorescent and transmission images were taken immediately after 10 min of incubation with the apoptosis/necrosis markers, without fixation. Images are representative from 3 independent experiments. All bars are 25 and 10 μm