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Fig. 7 | Molecular Cancer

Fig. 7

From: The P2X7 receptor regulates cell survival, migration and invasion of pancreatic ductal adenocarcinoma cells

Fig. 7

ATP stimulates pore formation. Yo-Pro-1 (2.5 μM) uptake was monitored after stimulation with 5 mM ATP alone, and in combination with a pre-inhibition (1 h) with AZ10606120 (10 μM) or A438079 (10 μM). Fluorescence of YoPro-1 (Ex. 490 nm/Em. 510 nm) was measured in Panc-1 (a, b), AsPC-1 (c, d) and BxPC-3 (e, f). a, c, e. Fluorescence values taken every 10 min for 60 min and normalized to the average of values before administration of ATP. Representative images of Yo-Pro-1 green fluorescence for control, 5 mM ATP and ionomycin stimulated cells (used as control to check Yo-Pro-1 efficacy) are shown as inserts for each cell lines. Note that BxPC-3 and AsPC-1 cells are more affected by ATP and ionomycin, compared to Panc-1 cells, which agrees with data in Fig. 3 and the fact that the pore inhibitor has greater effect. All bars are 100 μm. b, d, f. Values of normalized fluorescence at 60 min. The lines and bargraphs show data of four experiments (mean ± SEM); each run was carried out in triplicates. Significant differences P < 0.05 (*, #) and P < 0.001 (**, ##) from the respective control (#, ##) and with/without inhibitor (*, **) are indicated

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