Fig. 8

Effect of AZ10606120 on Panc-1 cells migration. Confluent Panc-1 cells, grown in 96-well plate were scratched with a 96-pin wound maker and treated with or without AZ10606120 (10 μM). a. The graph shows the percentage of open wound. Pictures were taken every 2 h. Images were analyzed with the IncuCyte software using the relative wound density option, which measures the density of the wound according to the density of the cell region. b. Percentage of wound still open after 24 h with and without inhibitor. The graphs show data of five experiments (mean ± SEM); each run was carried out in triplicates. c. Confluent Panc-1 cells, grown in the two chambers of an Ibidi silicon insert, were treated with or without AZ10606120 (10 μM). Phase contrast pictures were taken every hour and representative original images are shown at 0, 12 and 24 h after insert removal. d. Percentage of wound still open every hour with and without AZ10606120 (10 μM). Significant difference P < 0.05 (*) between control and inhibitor (d) is calculated on the slope of the curves. e. Percentage of wound still open after 24 h with and without inhibitor. In each picture the area was measured and normalized with the respective one at 0 h. In every experiment images were taken and area measured from three to seven fields. The graphs show data from three to five experiments (mean ± SEM). Significant difference calculated for wound open after 24 h