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Fig. 2 | Molecular Cancer

Fig. 2

From: A functional connectome: regulation of Wnt/TCF-dependent transcription by pairs of pathway activators

Fig. 2

Identification of novel Wnt regulators. a Dose-dependent induction of TCF dependent transcription in 7df3 cells by constitutively active LRP6 (∆NLRP). b and c Examples of the 45 inducers (b) and 96 inhibitors (c) of TCF-dependent transcription identified from a cDNA library screen of 9000 Xenopus tropicalis cDNAs (see Additional file 1: Figure S1). d Prune siRNA reduced h-Prune protein levels. FLAG-tagged h-Prune was transfected into MDA-MB231 breast cancer cells 24 h after transfection of either Renilla luciferase or Prune siRNA. Expression was detected with an anti-FLAG antibody. e Knockdown of Prune by siRNA reduced the level of active (de-phosphorylated) β-catenin in MDA-MB231 cells. Blot shown is representative of four separate experiments. f Levels of active β-catenin after siRNA transfection in 4 separate experiments were quantified. Knockdown of Prune reduces levels of de-phosphorylated β-catenin to a level that was not significantly different from the knockdown of β-catenin (Student’s T-test, p < 0.05)

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