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Fig. 3 | Molecular Cancer

Fig. 3

From: A functional connectome: regulation of Wnt/TCF-dependent transcription by pairs of pathway activators

Fig. 3

Transcription regulation in colon cancer cells and Xenopus laevis. a Example ‘inducer’ cDNAs effect on TopFlash reporter when co-transfected into HCT116 cells (see Additional file 4: Table S3 for complete list). Comparison with the mutant FopFlash reporter demonstrates high basal levels of β-catenin/TCF-dependent transcription. Results are from triplicate wells across different plates. Stars represent statistical significantly different values from TopFlash (Benjamini-Hochberg corrected p < 0.01). b Expression of a subset of ‘inducers’ activated transcription of the endogenous Wnt target genes Xnr3 and Siamois in animal cap assays. AC; uninjected animal caps. ODC; ornithine de-carboxylase control. c and d TopFlash reporter activity from a subset of cDNAs co-transfected into SW480 cells (c) or HCT116 cells (d). (see Additional file 3: Table S2 for complete a list). The data shown are from triplicate wells across different plates. Stars represent statistically significantly different values from TopFlash (Benjamini-Hochberg corrected p < 0.01)

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Molecular Cancer

ISSN: 1476-4598