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Fig. 4 | Molecular Cancer

Fig. 4

From: A functional connectome: regulation of Wnt/TCF-dependent transcription by pairs of pathway activators

Fig. 4

A network of functional interactions that modulate TCF-dependent transcription. a Reporter activity of cDNAs individually transfected into 7df3 cells. Green circles represent constitutively active versions of ‘core’ pathway members. The 5/45 cDNAs that were able to activate TCF dependent transcription to a level greater than background (Kolmogorov-Smirnov test, p < 0.01) are shown in blue. All other cDNAs were unable to activate TCF dependent transcription when individually transfected and are shown in black. ΔNLRP levels of activation were defined as 100. b Heat map displaying functional interactions. Red and blue squares indicate positive or negative interaction respectively. The opacity of the colour indicates the strength of the interaction. c Network visualisation of functional interactions. Positive (red) and suppressive (blue) interactions whose strength is represented by line opacity are displayed. Core nodes are coloured green, activator nodes are blue whilst enhancers are black. The core pathway members tend to be the most highly connected nodes. d and e Synergistic interactions were observed using promoter-reporters driven by the endogenous Wnt target genes Myc (d) or Siamois (e) in HEK293 cells. All combinations with the exception of Ras/CK1δ and the Siamois promoter showed (multiplicative) synergistic responses

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