Fig. 5

Functional interrogation of interacting pairs. a h-Prune synergy with HRAS was reduced following mutation of h-Prune catalytic residues (Prune 4Ddelta) [40]. Activation by cDNAs pairs (b) and inhibition by anti-Wnt signaling compounds (c) Compounds were added at 5 times their IC50 48 h after transfection. Luciferase assays were carried out 24 h later and were normalised to CMV-lacZ levels