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Fig. 2 | Molecular Cancer

Fig. 2

From: Impaired aldehyde dehydrogenase 1 subfamily member 2A-dependent retinoic acid signaling is related with a mesenchymal-like phenotype and an unfavorable prognosis of head and neck squamous cell carcinoma

Fig. 2

ALDH1A2 expression in HNSCC cell lines and impact of ATRA on tumor-related processes. a Western blot analysis with whole cell lysate demonstrates protein expression of ALDH1A2 and key regulators of RA signaling in FaDu and Cal27 cells. Detection of β-Actin served as control for quantity and quality of protein lysates. b Representative pictures of a colony-forming assay with Cal27 (upper panel) and FaDu cells (lower panel), which were cultured in the presence of ATRA at the indicated concentrations or DMSO as control. c The graph represents the relative survival fraction of Cal27 and FaDu cells following cultivation at the indicated concentration of ATRA. Data represent mean values ± SD of three independent experiments. d Relative number of BrdU-positive Cal27 cells following treatment with indicated concentrations of ATRA or DMSO as control. e Relative survival fraction of Cal27 cells after single irradiation with a dose of 2 Gy with or without ATRA treatment (3 μM). Bars represent mean values + SD of three independent replicates. * p-value ≤ 0.05, *** p-value ≤ 0.0005. f Representative pictures of an immunofluorescence staining for cleaved caspase 3 (green signal) with Cal27 cells, which were treated with indicated concentrations of ATRA or DMSO as control with or without irradiation (2 Gy or 5 Gy). Nuclear staining was done with H33324 (blue signal)

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Molecular Cancer

ISSN: 1476-4598