Fig. 2

PIM2 knockdown represses proliferation of Raji cells. a, c, e Western blots showing knockdown of PIM1, PIM2 and c-MYC after treatment with 5 mM IPTG in one representative clone each. In absence of IPTG, the different shRNAs were not expressed. b, d, f Cell numbers, as determined by MTT assay, of untreated and IPTG-treated cells were normalised to untreated cells. The means and SD of n independent experiments conducted in duplicate wells of six-well plates are shown. n = 3 for shPIM1, n = 3 for shPIM2, n = 2 for shMYC. g Means and SD of absolute cell numbers from (b, d and f) are plotted