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Fig. 4 | Molecular Cancer

Fig. 4

From: Loss of PIM2 enhances the anti-proliferative effect of the pan-PIM kinase inhibitor AZD1208 in non-Hodgkin lymphomas

Fig. 4

PIM1 and c-MYC bind to the GNL3 promoter. ChIP was carried out using chromatin from Raji cells and antibodies against PIM1 (a), c-MYC (b), Pol II CTD (c) or H3K9acS10p (d). Eluted gDNA was then subjected to qPCR using primer pairs in different regions of the GNL3 gene. a qPCR results were normalised to input and the GNL3 -3.5 kb region. Means and SD of six independent experiments are shown. b Results are shown normalised to input and the GNL3 -3.5 kb region. Means and sd of five independent experiments are shown for Raji cells, whereas one experiment was carried out in OCI-Ly10 cells. c Results are again normalised to input and the GNL3 -3.5 kb region. Means and SD of two independent experiments are shown for Raji cells, one experiment was done in OCI-Ly10 cells. d Results are normalised to H3 and the GNL3 -3.5 kb region. The means and SD of three independent experiments are shown

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