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Fig. 3 | Molecular Cancer

Fig. 3

From: Insulin-like growth factors are essential to prevent anoikis in oestrogen-responsive breast cancer cells: importance of the type I IGF receptor and PI3-kinase/Akt pathway

Fig. 3

Protective effect of IGF-1 against anchorage-dependent programmed cell death in breast cancer cells. MCF-7 (a and b), ZR-75 cells (a and b), and EFM-19 cells (a, b and c) were trypsinised, resuspended in serum-free medium alone or with 50 ng/ml IGF-1, placed in 35-mm-diameter poly-HEMA-coated wells and cultured for the indicated lengths of time. Cells were lysed and aliquots of protein analysed by western transfer for cleaved PARP and GAPDH. Representative western transfer images are shown (a). The amount of each protein was determined by densitometric scanning of X-ray films, corrected for GAPDH and is expressed as the percentage of the maximum value for each cell line (b). Asterisks show times at which cleaved PARP is statistically significantly less, in the presence of IGF-1 than in its absence (ANOVA, pā€‰<ā€‰0.001). EFM-19 cells were collected, fixed, permeabilised and incubated with FITC-conjugated antibody against activated caspase 3 and analysed in a FACSCalibur. Representative histograms are shown (c). The data was analysed with WinMDI which confirmed the protective effect of IGF-1 (pā€‰=ā€‰0.003)

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