Fig. 5
From: BALR-6 regulates cell growth and cell survival in B-lymphoblastic leukemia
BALR-6 knockdown leads to global differential expression of genes. a Unsupervised hierarchical gene clustering of differentially expressed genes upon BALR-6 siRNA mediated knockdown in RS4;11 cells (PPDE ≥ 95 %, fold change ≥ 1.5). qRT-PCR confirmation of BALR-6 knockdown shown below. Normalized to ACTIN. b, c Bar graphs of GO Slim classification enrichment analysis of differentially expressed genes by molecular function (b), and biological processes (c). d Enrichment analysis of transcription factor targets. Top ten transcription factors with a p-value ≤ 0.0001 are shown. SP1 had the highest number if dysregulated targets, highlighted in light green. For unknown transcription factors, binding site sequence is shown. e Disease association analysis by GLAD4U, revealing enrichment of genes associated to various malignancies, in particular, hematological malignancies (dark red). Diseases with a p-value ≤ 0.05 are shown.; PPDE, posterior probability of differential expression. Evaluations were made using a two-tailed T-test, p < 0.005 (**); p < 0.0005 (***)