Fig. 7

ZnT2 over-expression in MDA-MB-231 cells caused vesicular Zn sequestration and decreased cell viability. a Representative immunoblot of ZnT2-HA in ZnT2-over-expressing (ZnT2-OX) MDA-MB-231 cells. b Representative confocal images of FluoZin-3 fluorescence (green) in Mock-transfected and ZnT2-OX MDA-MB-231 cells. 60× magnification. Scale bar = 20 μm. c Data represent mean number of vesicles ± SEM, n = 8 cells/field from 3 different fields. Asterisk indicates a significant difference from Mock transfected controls, p < 0.05. d Data represent mean size of vesicles (μm) ± SEM, n = 8 cells/field from 3 different fields. Asterisk indicates a significant difference from Mock transfected controls, p < 0.05. e Data represent mean fluorescence intensity/cell ± SEM, n = 8 cells/field from 3 different fields. Asterisk indicates a significant difference from Mock transfected controls, p < 0.05. f Representative phase contrast images of Mock-transfected and ZnT2-OX MDA-MB-231 cells. 10× magnification. Scale bar = 100 μm. g Cell viability was assessed using the trypan blue exclusion assay. Data represent mean number of viable cells ± SD, n = 3 samples/genotype, from 3 independent experiments. Asterisk indicates a significant difference from Mock transfected controls, p < 0.05