Fig. 3

Role of actinomycin D on apoptosis. a and e Cell lines were treated with 10 nM of actinomycin D and protein expression was determined by Western Blot analysis at the given times. b and f Cell lines were treated with 10 nM of actinomycin D in presence or absence of 20 μM of Oph-QVD and cell viability was measured at indicated times. c and g Cell lines were treated with actinomycin D 10 nM in presence or absence of Oph-QVD 20 μM. After 24 h of treatment, the indicated protein expression levels were determined by Western blot analysis. d and h Cell lines were treated with 10 nM of actinomycin D and levels of the indicated RNAs were measured 24 h after treatment