Fig. 4
From: Deregulated expression of cryptochrome genes in human colorectal cancer
Evaluation of CRY1 and CRY2 protein levels in colon cancer cell lines. a Immunoblot detection of CRY1 and CRY2 protein in CaCo2, HCT116, HT29 and SW480 cells harvested 22 h after synchronization with serum shock; b-c) Immunoblot detection of CRY1 and CRY2 protein in CaCo2, HCT116, HT29 and SW480 cells with and without cryptochrome gene ectopic expression. Western blot analysis was performed to detect the protein expression levels of CRY1 and CRY2 upon transfection using anti-CRY1 and anti-CRY2 antibody as well as anti-FLAG antibody. β-Actin antibody was used as control. d basal p53 levels in CaCo2, HCT116, HT29 and SW480 cells; e) p53 levels upon CRY1 and CRY2 ectopic expression in the four colon cancer cell lines; bars, standard deviation (SD); *P < 0.05; **P < 0.01. Each western blot analysis was performed five times