Fig. 7

Evaluation of proliferation and cell cycle changes upon CRY1 and CRY2 ectopic expression in colon cancer cell lines. a MTT assays performed on CaCo2, HCT116, HT29 and SW480 cells transfected with CRY1 or CRY2 constructs. Wells containing transfected cells with reagents alone (Mock) were used as negative control; b) cell counts performed on CaCo2, HCT116, HT29 and SW480 cells transfected with CRY1 or CRY2 constructs. Wells containing transfected cells with reagents alone (Mock) were used as negative control; three biological replicates were prepared and each assayed in triplicate; the results were reported as mean ± standard deviation (SD); c) cell cycle analysis was performed three days after transfection on both attached and floating cells using the Cell-Cycle Test (BD Biosciences). Propidium Iodide stained cells (>20.000 events) were analyzed on FACSVerse flow cytometer (BD Biosciences); bars, standard deviation (SD); *P < 0.05; **P < 0.01; four biological replicates were each assayed in triplicate and results were expressed as mean ± SD