Fig. 8
From: Deregulated expression of cryptochrome genes in human colorectal cancer
Patterns of response to chemotherapeutic agents in colon cancer cell lines. a apoptotic and pre-apoptotic response to treatment with 5-fluorouracil (5-FU) or oxaliplatin (OXA) in CaCo2, HCT116, HT29 and SW480 cells; b) Effect of CRY1 or CRY2 ectopic expression on response to treatment with 5FU or OXA in colon cancer cell lines. The cell lines were treated with 1 μM, 5 μM and 10 μM of 5FU or OXA for 24 h at 37 °C in 5 % CO2 atmosphere 48 h after transfection with CRY1 or CRY2 constructs. Wells containing transfected cells with CRY1 or CRY2 constructs were used as negative control (Mock). Cell viability was determined by MTT assay. bars, standard deviation (SD); *P < 0.05; **P < 0.01; three biological replicates were each assayed in triplicate and results were expressed as mean ± SD