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Table 3 Summary of experimental data for the colon cancer cell lines examined

From: Deregulated expression of cryptochrome genes in human colorectal cancer

  CaCo2 HCT116 HT29 SW480
CRY1 endogenous mRNA levela ↑↑↑ ↑↑ ↑↑
CRY2 endogenous mRNA levela ↑↑↑ ↑↑ ↑↑
CRY1 endogenous protein levelb ↑↑↑ ↑↑
CRY2 endogenous protein levelb ↑↑ ↑↑↑
CRY1 ectopic protein level ↑↑↑
CRY2 ectopic protein level ↑↑↑
TP53 status mutated (Ø) wild-type mutated (GOF) mutated (GOF)
p53 endogenous protein level Ø ↑↑↑ ↑↑↑
p53 endogenous protein level upon CRY1 ectopic expression Ø ↑↑ = =
p53 endogenous protein level upon CRY2 ectopic expression Ø ↑↑ =
ARNTL endogenous mRNA levela ↑↑↑
c-MYC endogenous mRNA levela ↑↑↑ ↑↑↑
WEE endogenous mRNA levela ↑↑ ↑↑↑ ↑↑
Cell pre-apoptosis upon CRY1 ectopic expression = = ↓↓ ↓↓
Cell pre-apoptosis upon CRY2 ectopic expression = ↓↓
Cell apoptosis upon CRY1 ectopic expression = = ↓↓ ↓↓
Cell apoptosis upon CRY2 ectopic expression = ↓↓
Cell viability upon CRY1 ectopic expression = ↑↑ ↑↑ ↑↑
Cell viability upon CRY2 ectopic expression ↑↑ ↑↑ ↑↑
Cell cycle changes upon CRY1 ectopic expression S↑ G2/M= S↑ G2/M= S↑ G2/M↓ S = G2/M↑
Cell cycle changes upon CRY2 ectopic expression S↑ G2/M= S↑ G2/M= S↑ G2/M↓ S↑ G2/M=
Cell pre-apoptosis upon treatment with OXA ↑↑↑ ↑↑ ↑↑ ↑↑
Cell pre-apoptosis upon treatment with 5FU ↑↑↑ = ↑↑ ↑↑
Cell apoptosis upon treatment with OXA ↑↑↑ ↑↑ ↑↑ ↑↑
Cell apoptosis upon treatment with 5FU ↑↑↑ = ↑↑ ↑↑
Cell viability upon CRY1 ectopic expression + OXAc =
Cell viability upon CRY2 ectopic expression + OXAc = =
Cell viability upon CRY1 ectopic expression + 5FUc =
Cell viability upon CRY2 ectopic expression + 5FUc = =
  1. acells harvested 21 h after synchronization with serum shock; bcells harvested 22 h after synchronization with serum shock; Ø protein not detected, GOF gain of function; ccompared to mock transfected cells