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Fig. 2 | Molecular Cancer

Fig. 2

From: miR-508-3p concordantly silences NFKB1 and RELA to inactivate canonical NF-κB signaling in gastric carcinogenesis

Fig. 2

NFKB1 and RELA knockdown exerts tumor suppressor function in GC cells. a Transfection with siNFKB1 or siRELA decreased the mRNA expression of NFKB1 and RELA respectively in MKN28, MGC-803 and SGC-7901 cells. b 6-day MTT assays revealed NFKB1 or RELA knockdown significantly suppressed proliferation rate of GC cells (**, P < 0.001). c siNFKB1 or siRELA decreased monolayer colony formation in MKN28, MGC-803 and SGC-7901 cells (*, P < 0.05; **, P < 0.001). The experiments were performed in triplicate wells to get SDs. d NFKB1 or RELA knockdown inhibited GC cell invasion (*, P < 0.05; **, P < 0.001). Three random vision fields were selected for invaded cell counting to get SDs. e The GC cell migration abilities were suppressed by siNFKB1 or siRELA (*, P < 0.05; **, P < 0.001). The cells were counted in three random vision fields to get SDs. f Flow cytometry analysis of NFKB1 or RELA knockdown transfectants together with scramble siRNA transfectants as control. Two independent experiments were performed and the representative one was shown in the bar chart. g Effects of siNFKB1 and siRELA on the induction of early apoptosis (Annexin V-FITC and PI double-staining). The cell population with early apoptosis was shown in the lower right of each treatment. siNFKB1 or siRELA induced early apoptosis compared with siScramble control after 20-h transfection (*, P < 0.05, **, P < 0.001). h Upper, Western blot analysis of NFKB1, p21, p27, cleaved-PRAP and p-Rb after siNFKB1 transfection; Lower, the Western blot result of RELA, cleaved-PRAP and p-Rb in the transfectants of siRELA. i siNFKB1 and siRELA formed smaller xenografts than siScramble using MGC-803 cells in a 28-day inoculation and the elevated cleaved-PARP was detected in siNFKB1 and siRELA xenografts

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